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P. cunningtonii vs P. elpatiewskyi

Morphological features: These two species are not so easy to tell apart: they are of the same size and occur in the water at the same time. Examining their thecal plate structure allows distinction between them: the plates differ in number and arrangement, especially when observed from above (Plate 1). However, without an SEM the plates are usually seen only on empty . To identify the living cells under the light microscope we examine (Plates 1, 2, 3):
(1) cell shape – P. elpatiewskyi is angular whereas P. cunningtonii is smooth and rounded, especially its quinquecuspidata forma;
(2) spines on P. cunningtonii has 1-4 spines, more or less of the same size and evenly spaced. P. elpatiewskyi has 2-3 main spines and to the side of one of them a “brush” of smaller spines.
(3) hypotheca base - in P. elpatiewskyi it is flat to concave whereas that of P. cunningtonii is rounded.
P. cunningtonii vs P. elpatiewskyi — figure 1 (from source)

Additional figures

Plate 1. Distinguishing between Parvodinium elpatiewskyi (left) and P. cunningtonii (right). Top: epitheca of both species showing the different number and arrangement of the thecal plates of the epitheca. Bottom: P. elpatiewskyi (left): angular shape, flat bottom, a “brush” of spines on one side of hypotheca; P. Cunningtonii (right): rounded shape, rounded base of the hypotheca, two distinct and separated spines.
Plate 2. Distinguishing between Parvodinium elpatiewskyi and P. cunningtonii: light microscopy.
Plate 3. Distinguishing between Parvodinium elpatiewskyi and P. cunningtonii: SEM microscopy. Note punctae along plate margins in P. elpatiewskyi that are absent in P. cunningtonii. Also note concave shape of plates only in P. elpatiewskyi.

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